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lipid iron assay kit  (Dojindo Labs)


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    Dojindo Labs lipid iron assay kit
    Lipid Iron Assay Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lipid+iron+assay+kit/pm41436912-62-12-19?v=Dojindo+Labs
    Average 96 stars, based on 128 article reviews
    lipid iron assay kit - by Bioz Stars, 2026-08
    96/100 stars

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    lipid peroxidation assay kit  (Elabscience Biotechnology)
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    Validation of NSC777201-induced cell death in colorectal cancer (CRC) cells. (A) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29), 150 µM 5-FU, or 50 µM oxaliplatin for 24 h. Protein expression levels were analyzed by Western blotting. (B) HCT116 and HT29 cells were treated with 6, 8, or 10 µM of NSC777201 for 6 h. Protein expression levels were assessed by Western blotting. (C) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and then stained with Cyto-ID Autophagy Detection Reagent. The Cyto-ID fluorescence was observed under a fluorescence microscope. Scale bar = 50 μm. (D) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h. Protein expression levels were analyzed by Western blotting. (E) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and a lipid <t>peroxidation</t> assay was performed. Scale bar = 100 μm. (F) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and then stained with the FerroOrange probe. Fluorescence was observed under a fluorescence microscope. Scale bar = 100 μm
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    Validation of NSC777201-induced cell death in colorectal cancer (CRC) cells. (A) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29), 150 µM 5-FU, or 50 µM oxaliplatin for 24 h. Protein expression levels were analyzed by Western blotting. (B) HCT116 and HT29 cells were treated with 6, 8, or 10 µM of NSC777201 for 6 h. Protein expression levels were assessed by Western blotting. (C) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and then stained with Cyto-ID Autophagy Detection Reagent. The Cyto-ID fluorescence was observed under a fluorescence microscope. Scale bar = 50 μm. (D) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h. Protein expression levels were analyzed by Western blotting. (E) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and a lipid <t>peroxidation</t> assay was performed. Scale bar = 100 μm. (F) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and then stained with the FerroOrange probe. Fluorescence was observed under a fluorescence microscope. Scale bar = 100 μm
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    Validation of NSC777201-induced cell death in colorectal cancer (CRC) cells. (A) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29), 150 µM 5-FU, or 50 µM oxaliplatin for 24 h. Protein expression levels were analyzed by Western blotting. (B) HCT116 and HT29 cells were treated with 6, 8, or 10 µM of NSC777201 for 6 h. Protein expression levels were assessed by Western blotting. (C) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and then stained with Cyto-ID Autophagy Detection Reagent. The Cyto-ID fluorescence was observed under a fluorescence microscope. Scale bar = 50 μm. (D) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h. Protein expression levels were analyzed by Western blotting. (E) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and a lipid <t>peroxidation</t> assay was performed. Scale bar = 100 μm. (F) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and then stained with the FerroOrange probe. Fluorescence was observed under a fluorescence microscope. Scale bar = 100 μm
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    assays iron assay kit leagene biotechnology tc1015 lipid peroxidation mda assay kit beyotime s0131s experimental models - by Bioz Stars, 2026-08
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    Validation of NSC777201-induced cell death in colorectal cancer (CRC) cells. (A) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29), 150 µM 5-FU, or 50 µM oxaliplatin for 24 h. Protein expression levels were analyzed by Western blotting. (B) HCT116 and HT29 cells were treated with 6, 8, or 10 µM of NSC777201 for 6 h. Protein expression levels were assessed by Western blotting. (C) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and then stained with Cyto-ID Autophagy Detection Reagent. The Cyto-ID fluorescence was observed under a fluorescence microscope. Scale bar = 50 μm. (D) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h. Protein expression levels were analyzed by Western blotting. (E) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and a lipid <t>peroxidation</t> assay was performed. Scale bar = 100 μm. (F) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and then stained with the FerroOrange probe. Fluorescence was observed under a fluorescence microscope. Scale bar = 100 μm
    Iron Assay Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 1 article reviews
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    Validation of NSC777201-induced cell death in colorectal cancer (CRC) cells. (A) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29), 150 µM 5-FU, or 50 µM oxaliplatin for 24 h. Protein expression levels were analyzed by Western blotting. (B) HCT116 and HT29 cells were treated with 6, 8, or 10 µM of NSC777201 for 6 h. Protein expression levels were assessed by Western blotting. (C) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and then stained with Cyto-ID Autophagy Detection Reagent. The Cyto-ID fluorescence was observed under a fluorescence microscope. Scale bar = 50 μm. (D) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h. Protein expression levels were analyzed by Western blotting. (E) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and a lipid peroxidation assay was performed. Scale bar = 100 μm. (F) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and then stained with the FerroOrange probe. Fluorescence was observed under a fluorescence microscope. Scale bar = 100 μm

    Journal: Cancer Cell International

    Article Title: NSC777201 exhibits anticancer activity against colorectal cancer cells by inducing multiple types of cell death

    doi: 10.1186/s12935-025-04146-6

    Figure Lengend Snippet: Validation of NSC777201-induced cell death in colorectal cancer (CRC) cells. (A) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29), 150 µM 5-FU, or 50 µM oxaliplatin for 24 h. Protein expression levels were analyzed by Western blotting. (B) HCT116 and HT29 cells were treated with 6, 8, or 10 µM of NSC777201 for 6 h. Protein expression levels were assessed by Western blotting. (C) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and then stained with Cyto-ID Autophagy Detection Reagent. The Cyto-ID fluorescence was observed under a fluorescence microscope. Scale bar = 50 μm. (D) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h. Protein expression levels were analyzed by Western blotting. (E) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and a lipid peroxidation assay was performed. Scale bar = 100 μm. (F) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and then stained with the FerroOrange probe. Fluorescence was observed under a fluorescence microscope. Scale bar = 100 μm

    Article Snippet: A lipid peroxidation assay kit (E-BC-K880-M) was purchased from Elabscience (Houston, TX, USA).

    Techniques: Biomarker Discovery, Expressing, Western Blot, Staining, Fluorescence, Microscopy, Peroxidation Assay

    Impact of exogenous 7-dehydrocholesterol (7-DHC) on NSC777201-induced cytotoxicity and lipid peroxidation. (A) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and a real-time qPCR was performed to measure gene expressions. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (B) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and an ELISA was performed to measure intracellular 7-DHC levels. *** p < 0.001. (C) HCT116 and HT29 cells were treated with indicated doses of NSC777201 for 24 h, with or without 30 µM 7-DHC. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (D) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) with or without 30 µM 7-DHC for 6 h, and a lipid peroxidation assay was performed. Scale bar = 100 μm

    Journal: Cancer Cell International

    Article Title: NSC777201 exhibits anticancer activity against colorectal cancer cells by inducing multiple types of cell death

    doi: 10.1186/s12935-025-04146-6

    Figure Lengend Snippet: Impact of exogenous 7-dehydrocholesterol (7-DHC) on NSC777201-induced cytotoxicity and lipid peroxidation. (A) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and a real-time qPCR was performed to measure gene expressions. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (B) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) for 6 h, and an ELISA was performed to measure intracellular 7-DHC levels. *** p < 0.001. (C) HCT116 and HT29 cells were treated with indicated doses of NSC777201 for 24 h, with or without 30 µM 7-DHC. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (D) HCT116 and HT29 cells were treated with NSC777201 (6 µM for HCT116 and 8 µM for HT29) with or without 30 µM 7-DHC for 6 h, and a lipid peroxidation assay was performed. Scale bar = 100 μm

    Article Snippet: A lipid peroxidation assay kit (E-BC-K880-M) was purchased from Elabscience (Houston, TX, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Peroxidation Assay